DARPP-32 Antibody [Unconjugated]

Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DARPP-32 at approximately 32 - 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DARPP-32 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

DARPP-32 was detected in perfusion fixed frozen sections of mouse brain (caudate putamen) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

DARPP-32 was detected in perfusion fixed frozen sections of rat brain (globus pallidus) using Goat Anti-Human/Mouse/Rat DARPP-32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6259) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DARPP-32 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human DARPP‑32
  Arg51-Ala204
  Accession # Q9UD71
• Specificity: Detects human, mouse, and rat DARPP‑32 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP1R1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DARPP-32 Antibody [Unconjugated]

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARPP-32 Antibody (AF6259)(6)

Publications using AF6259

• Guadalupi, L;Vanni, V;Balletta, S;Caioli, S;De Vito, F;Fresegna, D;Sanna, K;Nencini, M;Donninelli, G;Volpe, E;Mariani, F;Battistini, L;Stampanoni Bassi, M;Gilio, L;Bruno, A;Dolcetti, E;Buttari, F;Mandolesi, G;Centonze, D;Musella, A; Interleukin-9 protects from microglia- and TNF-mediated synaptotoxicity in experimental multiple sclerosis Journal of neuroinflammation 2024-05-14 [PMID: 38745307] (Immunohistochemistry, Mouse)
• Ayon-Olivas, M;Wolf, D;Andreska, T;Granado, N;Lüningschrör, P;Ip, CW;Moratalla, R;Sendtner, M; Dopaminergic Input Regulates the Sensitivity of Indirect Pathway Striatal Spiny Neurons to Brain-Derived Neurotrophic Factor Biology 2023-10-23 [PMID: 37887070] (Western Blot, Mouse)
• Giulia Ponterio, Gaia Faustini, Ilham El Atiallah, Giuseppe Sciamanna, Maria Meringolo, Annalisa Tassone et al. Alpha‐Synuclein is Involved in DYT1 Dystonia Striatal Synaptic Dysfunction Movement Disorders 2022-05-01 [PMID: 35420219]
• Helseth AR, Hernandez-Martinez R, Hall VL et al. Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice Science (New York, N.Y.) 4/23/2021 [PMID: 33888613]
• Freja K. Ekman, David S. Ojala, Maroof M. Adil, Paola A. Lopez, David V. Schaffer, Thomas Gaj CRISPR-Cas9-Mediated Genome Editing Increases Lifespan and Improves Motor Deficits in a Huntington’s Disease Mouse Model Molecular Therapy - Nucleic Acids 2019-09-06 [PMID: 31465962] (Immunohistochemistry, Transgenic Mouse)
• M Maltese, J Stanic, A Tassone, G Sciamanna, G Ponterio, V Vanni, G Martella, P Imbriani, P Bonsi, NB Mercuri, F Gardoni, A Pisani Early structural and functional plasticity alterations in a susceptibility period of DYT1 dystonia mouse striatum Elife, 2018-03-05;7(0):. 2018-03-05 [PMID: 29504938] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: PPP1R1B
• Uniprot:
  • Q9UD71(Human)