DARPP-32 Antibody (SU0329)

Images

 
Western Blot: DARPP-32 Antibody (SU0329) [NBP2-67462] - Western blot analysis of DARPP-32 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. ...read more
Immunocytochemistry/ Immunofluorescence: DARPP-32 Antibody (SU0329) [NBP2-67462] - Staining DARPP32 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: DARPP-32 Antibody (SU0329) [NBP2-67462] - Analysis of paraffin-embedded rat brain tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Flow Cytometry: DARPP-32 Antibody (SU0329) [NBP2-67462] - Analysis of Hela cells with DARPP32 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Western Blot: DARPP-32 Antibody (SU0329) [NBP2-67462] - Analysis of DARPP32 on mouse brain lysates using anti-DARPP32 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: DARPP-32 Antibody (SU0329) [NBP2-67462] - Staining DARPP32 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: DARPP-32 Antibody (SU0329) [NBP2-67462] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: DARPP-32 Antibody (SU0329) [NBP2-67462] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DARPP32 antibody. Counter stained with hematoxylin.

Product Details

Summary
Product Discontinued
View other related DARPP-32 Primary Antibodies

Order Details


    • Catalog Number
      NBP2-67462
    • Availability
      Product Discontinued

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DARPP-32 Antibody (SU0329) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within Human DARPP-32 aa 1-94 / 204. (SwissProt: Q9UD71 Human; SwissProt: Q60829 Mouse; SwissProt: Q6J4I0 Rat)
Localization
Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PPP1R1B
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for DARPP-32 Antibody (SU0329)

  • DARPP32
  • DARPP-32
  • PPP1R1B
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
  • regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPP1R1B