DAP1 Antibody (E59)

Product Discontinued

DAP1 Antibody (E59) Summary

• Immunogen: A synthetic peptide corresponding to C-terminus of human DAP-1 was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DAP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 11kDa.

Reactivity Notes

Reacts with: Human Does not react with Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for DAP1 Antibody (E59)

• DAP1
• DAP-1
• death-associated protein 1
• death-associated protein
• MGC99796

Background

Death associated protein 1 (DAP-1) is a 15-kDa protein that functions as a positive mediator of cell death initiated by interferon (IFN-) gamma (1,2). DAP-1 is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). It is localized to the cytoplasm, but the detailed mechanism of its pro-apoptotic function is unclear. DAP-1 and DAP kinase, a unique, 160-kDa calmodulin-dependent serine/threonine kinase, are both unique candidates for positive mediators of programmed cell death induced by IFN-gamma (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for DAP1 Antibody (NB110-56925). (Showing 1 - 1 of 1 FAQ).

1. I have a homology question. If you could get me the sequence of the immunogen that would be fantastic but, if not, a percent homology between the immunogen and the protein with GenBank Accession AFE84783 would work fine.
   • The peptide maps between amino acids 75 - 102, at the C-terminus of human DAP1 (UniProt: P51397). The exact immunogen shares 55% identity with GenBank Accession AFE84783, so I would not predict cross-reactivity. It is not guaranteed without testing.

Bioinformatics

• Gene Symbol: DAP
• Entrez:
  • 1611(Human)
  • 223453(Mouse)
  • 64322(Rat)
• Uniprot:
  • P51397(Human)