Recombinant Human Cystatin-9 His Protein

SDS-Page: Recombinant Human Cystatin-9 Protein [NBP1-78812] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human Cystatin-9 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-159 of Human Cystatin-9
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MWCSEEEMGG NNKIVQDPMF LATVEFALNT FNVQSKEEHA YRLLRVLSSW REDSMDRKWR GKMVFSMNLQ LRQTVCRKFE DDIDNCPFQE SLELNNVRQG ISFPQVHSCG CCMGCGVGTG AADKAIPRDK GK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CST9
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 17.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Cystatin-9 His Protein

• cystatin 9 (testatin)

Background

Cystatin 9, also known as CST9, is a member of the CRES (cystatin-related epididymal spermatogenic) subfamily. The CRES (cystatin-related epididymal spermatogenic) protein defines a newsubgroup in the family 2 cystatins of the cystatin superfamily. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. CST9 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas. CST9 is thought to play a role in hematopoietic differentiation or inflammation. It has also been shown to be upregulated by LPS in some cancer cell lines, such as promyelocytic leukemia (HL-60) and myelomonocytic leukemia. Recombinant human CST9 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CST9