Recombinant Human CYP4Z1 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human CYP4Z1 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-505 of Human CYP4Z1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MEPSWLQELMAHPFLLLILLCMSLLLFQVIRLYQRRRWMIRALHLFPAPPAHWFYGHKEFYPVKEFEVYHKLMEKYPCAVPLWVGPFTMFFSVHDPDYAKILLKRQDPKSAVSHKILESWVGRGLVTLDGSKWKKHRQIVKPGFNISILKIFITMMSESVRMMLNKWEEHIAQNSRLELFQHVSLMTLDSIMKCAFSHQGSIQLDSTLDSYLKAVFNLSKISNQRMNNFLHHNDLVFKFSSQGQIFSKFNQELHQFTEKVIQDRKESLKDKLKQDTTQKRRWDFLDILLSAKSENTKDFSEADLQAEVKTFMFAGHDTTSSAISWILYCLAKYPEHQQRCRDEIRELLGDGSSITWEHLSQMPYTTMCIKECLRLYAPVVNISRLLDKPITFPDGRSLPAGITVFINIWALHHNPYFWEDPQVFNPLRFSRENSEKIHPYAFIPFSAGLRNCIGQHFAIIECKVAVALTLLRFKLAPDHSRPPQPVRQVVLKSKNGIHVFAKKVC

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: CYP4Z1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 85.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CYP4Z1 GST (N-Term) Protein

• CYP4A20
• CYPIVZ1
• cytochrome P450 4Z1
• cytochrome P450, family 4, subfamily Z, polypeptide 1
• EC 1.14.14.1

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 1p33. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CYP4Z1 Recombinant Protein (H00199974-P01)(4)

Publications using H00199974-P01

• Yousef M. Al-saraireh, Fatemah O. F. O. Alshammari, Anas O. Satari, Yanal S. Al-mahdy, Ghadeer H. Almuhaisen, Omar H. Abu-azzam, Ala N. Uwais, Seham M. Abufraijeh, Ahlam M. Al-Kharabsheh, Sa’ed M. Al-dalain, Aiman Al-Qtaitat, Fatima Al-Tarawneh, Jehad M. Al Shuneigat, Sameeh A. Al-Sarayreh, Udo Jeschke Cytochrome 4Z1 Expression Connotes Unfavorable Prognosis in Ovarian Cancers Medicina 2022-09-13 [PMID: 36143940]
• Al-Saraireh YM, Alshammari FOFO, Youssef AMM Et al. Cytochrome 4Z1 Expression is Associated with Poor Prognosis in Colon Cancer Patients OncoTargets and therapy 2021-11-12 [PMID: 34803385] (B/N)
• Al-Saraireh YM, Alshammari FOFO, Youssef AMM Et al. Cytochrome 4Z1 Expression is Associated with Unfavorable Survival in Triple-Negative Breast Cancers Breast cancer (Dove Medical Press) 2021-10-05 [PMID: 34675653] (B/N)
• Yousef M. Al-saraireh, Fatemah O. F. O. Alshammari, Ahmed M. M. Youssef, Yahya M. Al-sarayra, Renata A. Al-saraireh, Ghadeer H. Al-muhaisen, Yanal S. Al-mahdy, Ahlam M. Al-Kharabsheh, Seham M. Abufraijeh, Hamzeh Mohammad Alrawashdeh Cytochrome 4Z1 Expression Is Correlated with Poor Prognosis in Patients with Cervical Cancer Current Oncology 2021-09-16 [PMID: 34590601]

Bioinformatics

• Gene Symbol: CYP4Z1