Cyclophilin B Antibody (M019)

Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.

Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody (M019) [NBP3-23271] - Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (NBP3-23271). The antibody was detected using goat anti-mouse DyLight(R) 594.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: M019
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cyclophilin B Antibody (M019) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M019 was generated from a proprietary antigen related to cyclophilin B from the MCF7 breast cancer cell line. [UniProt# P23284]
• Specificity: Clone M019 detects a 20 kDa band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunoprecipitation 1:100
  • Western Blot 1:1000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Cyclophilin B Antibody (M019)

• Cyclophilin B
• cyclophilin-like protein
• CYPB
• CYPBEC 5.2.1.8
• CYP-S1
• MGC2224
• OI9MGC14109
• peptidyl-prolyl cis-trans isomerase B
• peptidylprolyl isomerase B (cyclophilin B)
• PPIase B
• PPIB
• Rotamase B
• S-cyclophilin
• SCYLP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPIB