Cyclophilin A Antibody

Western Blot: Cyclophilin A Antibody [NB100-2467] - Staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cyclophilin A Antibody Summary

• Description: Novus Biologicals Goat Cyclophilin A Antibody (NB100-2467) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cyclophilin A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ERFGSRNGKTSKK corresponding to internal region according to NP_066953.1.
• Specificity: This product is expected to also recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPIA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 18 kDa band observed in human lymph node lysates (calculated MW 18.0 kDa band according to NP_066953.1),
• Control:

  Cyclophilin A Overexpression Lysate
  Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cyclophilin A Antibody

• Cyclophilin A
• Cyclosporin A-binding protein
• CYPA
• CYPAMGC12404
• CYPH
• EC 5.2.1.8
• MGC117158
• MGC23397
• peptidyl-prolyl cis-trans isomerase A
• peptidylprolyl isomerase A (cyclophilin A)
• PPIA
• PPIase A
• Rotamase A
• Rotamase
• T cell cyclophilin

Background

Gene ID(s): 5478 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin A Antibody (NB100-2467)(1)

Publication using NB100-2467

• Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci U S A 2005-10-11 [PMID: 16203999]

Bioinformatics

• Gene Symbol: PPIA
• Entrez:
  • 5478(Human)
• Uniprot:
  • NP_066953.1(Human)