Cyclophilin A Antibody (1F4-1B5) - Azide and BSA Free

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - PPIA monoclonal antibody (M01), clone 1F4-1B5 Analysis of PPIA expression in Jurkat.

Western Blot: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - Analysis of PPIA expression in transfected 293T cell line by PPIA monoclonal antibody (M01), clone 1F4-1B5.Lane 1: PPIA transfected lysate(18 KDa).Lane 2: Non-transfected lysate.

ELISA: Cyclophilin A Antibody (1F4-1B5) [H00005478-M01] - Detection limit for recombinant GST tagged PPIA is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1F4-1B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cyclophilin A Antibody (1F4-1B5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPIA (AAH00689.1, 1 a.a. - 165 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE
• Specificity: PPIA - peptidylprolyl isomerase A (cyclophilin A)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPIA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cyclophilin A Antibody (1F4-1B5) - Azide and BSA Free

• Cyclophilin A
• Cyclosporin A-binding protein
• CYPA
• CYPAMGC12404
• CYPH
• EC 5.2.1.8
• MGC117158
• MGC23397
• peptidyl-prolyl cis-trans isomerase A
• peptidylprolyl isomerase A (cyclophilin A)
• PPIA
• PPIase A
• Rotamase A
• Rotamase
• T cell cyclophilin

Background

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclophilin A Antibody (H00005478-M01)(4)

Publications using H00005478-M01

• Satkunanathan S, Wheeler J, Thorpe R, Zhao Y. Establishment of a Novel Cell Line for the Enhanced Production of Recombinant AAV Vectors for Gene Therapy. Hum Gene Ther. 2014-07-29 [PMID: 25072415]
• Deng FY, Lei SF, Zhang Y et al. Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans. Mol Cell Proteomics. 2011-08-04 [PMID: 21817168]
• Rho JH, Roehrl MH, Wang JY. Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma. J Proteome Res 8(12):5610-8. 2009-12-01 [PMID: 19848416]
• Sedelaar JP, Isaacs JT. Tissue culture media supplemented with 10% fetal calf serum contains a castrate level of testosterone. Prostate 69(16):1724-9. 2009-12-01 [PMID: 19676093]

Bioinformatics

• Gene Symbol: PPIA
• Entrez:
  • 5478(Human)
• OMIM:
  • 123840(Human)
• Uniprot:
  • AAH00689(Human)