Cullin 2 Antibody - Pre-diluted

Product Discontinued

Cullin 2 Antibody - Pre-diluted Summary

• Immunogen: Synthetic peptide (Human) (C terminal).
• Localization: Nuclear
• Specificity: Cullin 2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin Neat
• Application Notes: IHC-P: Ready diluted. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
• Control:

  Cullin 2 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human and Mouse.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Cullin 2 Antibody - Pre-diluted

• CUL2
• CUL-2
• Cullin 2
• cullin-2
• MGC131970

Background

Cullin 2 is a member of the family of human Cullin genes (CUL1, 2, 3, 4a, 4b and 5) homologous to the S. cerevisiae cdc53 gene. It is a component of E3 ubiquitin ligase complexes, including the inactive transcriptional elongation complex SIII, which mediates the ubiquitination of hypoxia-inducible factor (HIF). SIII is formed by three subunits: Elongin C, Elongin B and VHL. Cullin 2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CUL2
• Entrez:
  • 8453(Human)
  • 71745(Mouse)