CSN1 Antibody

Western Blot: CSN1 Antibody [NB100-1237] - Human Testis lysate (RIPA buffer, 30 ug total protein). Antibody at 0.5 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Tonsil. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: CSN1 Antibody [NB100-1237] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CSN1 Antibody Summary

• Immunogen: Peptide with sequence C-PANSQSRMSTNM corresponding to C-Terminus according to NP_004118.3.
• Specificity: This antibody is expected to recognise isoform 1 (NP_997657.1) and isoform 2 (NP_004118.3).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 ug/mL
• Application Notes: WB: Approx. 55 kDa band observed in human testis lysate (predicted MW of 58 kDa band according to NP_004118).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSN1 Antibody

• COP9 signalosome complex subunit 1
• COPS1JAB1-containing signalosome subunit 1
• CSN1MGC71287
• G protein pathway suppressor 1SGN1
• GPS-1
• Protein MFH
• Signalosome subunit 1

Background

This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. The encoded protein shares significant similarity with Arabidopsis FUS6, which is a regulator of light-mediated signal transduction in plant cells. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSN1 Antibody (NB100-1237)(1)

Publication using NB100-1237

• Spain BH, Bowdish KS, Pacal AR et al. Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells. Mol Cell Biol 1996-12-01 [PMID: 8943324]

Bioinformatics

• Gene Symbol: GPS1
• Entrez:
  • 2873(Human)
  • 209318(Mouse)
  • 117039(Rat)
• Uniprot:
  • NP_004118.(Human)