CSE1L/CAS/Exportin-2 Antibody (JU34-33)

Western Blot: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution.Positive control:Lane 1: Mouse testis Lane 2: SiHaLane 3: SK-BR-3 Lane 4: PC-3M

Immunocytochemistry/Immunofluorescence: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Analysis of paraffin-embedded mouse testis tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.

Flow Cytometry: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Analysis of LOVO cells with Cellular Apoptosis Susceptibility antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody (JU34-33) [NBP2-75451] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.

Product Discontinued

CSE1L/CAS/Exportin-2 Antibody (JU34-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CSE1L/CAS/Exportin-2 aa 860-971 / 971. (SwissProt: P55060 Human; SwissProt: Q9ERK4 Mouse)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSE1L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CSE1L/CAS/Exportin-2 Antibody (JU34-33)

• CAS
• CASMGC117283
• Cellular apoptosis susceptibility protein
• chromosome segregation 1 (yeast homolog)-like
• Chromosome segregation 1-like protein
• CSE1 chromosome segregation 1-like (yeast)
• CSE1
• CSE1L
• exp2
• exportin-2
• Importin-alpha re-exporter
• MGC130037
• XPO2
• XPO2MGC130036

Background

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSE1L/CAS/Exportin-2 Antibody (NBP2-75451)(1)

Publication using NBP2-75451

• Sarcar B, Fang B, Izumi V et al. A comparative proteomics analysis identified differentially expressed proteins in pancreatic cancer-associated stellate cell small extracellular vesicles Molecular & Cellular Proteomics 2022-11-01 [PMID: 36332889] (WB, Human)

Bioinformatics

• Gene Symbol: CSE1L