CRLR Antibody (998820) [Unconjugated]

CRLR was detected in immersion fixed KG-1 human acute myelogenous leukemia cell line using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CRLR was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human CRLR Monoclonal Antibody (Catalog # MAB10044) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 998820
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CRLR Antibody (998820) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human CALCRL
  Accession # Q16602
• Specificity: Detects human CALCRL in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRLR Antibody (998820) [Unconjugated]

• calcitonin gene-related peptide type 1 receptor
• calcitonin receptor-like
• CALCRL
• CGRP type 1 receptor
• CGRPR
• CGRPRCRLRCalcitonin receptor-like receptor
• CRLR

Background

The calcitonin receptor-like receptor (CRLR), a class B GPCR, forms a heterodimer with receptor activity-modifying protein 2 (RAMP2), and serves as the adrenomedullin (AM) receptor to control neovascularization, while CRLR and RAMP1 form the calcitonin gene-related peptide (CGRP) receptor. CALCRL is pharmacologically identical to CGRP receptor-1, a putative molecular target of CGRP and adrenomedullin. CALCRL is predominantly expressed in the lung and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRLR Antibody (MAB10044)(1)

Publication using MAB10044

• ?erban, RE;Boldeanu, MV;Florescu, DN;Ionescu, M;?erb?nescu, MS;Boldeanu, L;Florescu, MM;Stepan, MD;Obleag?, VC;Constantin, C;Popescu, DM;Streba, CT;Vere, CC; Comparison between Substance P and Calcitonin Gene-Related Peptide and Their Receptors in Colorectal Adenocarcinoma Journal of clinical medicine 2024-09-22 [PMID: 39337103] (Immunohistochemistry, Human)

Bioinformatics

• Uniprot:
  • Q16602()