Creatine Kinase, Muscle/CKMM Antibody [Unconjugated]

Simple Western lane view shows lysates of Human Heart, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/CKMM at approximately 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Creatine Kinase MM/CKMM Antigen Affinity-purified Polyclonal Antibody (Catalog # G-113-C). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Creatine Kinase, Muscle/CKMM Antibody [Unconjugated] Summary

• Immunogen: Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CKM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄, NaCl and Sodium Azide with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute with sterile PBS. If 0.5 mL of PBS is used, the antibody concentration will be 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Creatine Kinase, Muscle/CKMM Antibody [Unconjugated]

• CKM
• CKMM
• CKMMcreatine kinase M-type
• Creatine Kinase Isoenzyme
• Creatine kinase M chain
• Creatine Kinase MM
• creatine kinase, muscle
• creatine kinase-M
• EC 2.7.3
• EC 2.7.3.2
• M-CK

Background

Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CKM