Creatine Kinase, Muscle/CKMM Antibody (492731) [Unconjugated]

Images

 
Western blot shows lysate of human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Creatine Kinase MM/CKMM Monoclonal Antibody (Catalog # MAB5564) followed by HRP-conjugated Anti-Mouse IgG ...read more
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Creatine Kinase MM/CKMM at approximately 51 kDa (as indicated) using 1 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
492731
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Creatine Kinase, Muscle/CKMM Antibody (492731) [Unconjugated] Summary

Immunogen
Human cardiac muscle-derived Creatine Kinase, Muscle/CKMM
Specificity
Detects human Creatine Kinase, Muscle/CKMM in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CKM
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Creatine Kinase, Muscle/CKMM Antibody (492731) [Unconjugated]

  • CKM
  • CKMM
  • CKMMcreatine kinase M-type
  • Creatine Kinase Isoenzyme
  • Creatine kinase M chain
  • Creatine Kinase MM
  • creatine kinase, muscle
  • creatine kinase-M
  • EC 2.7.3
  • EC 2.7.3.2
  • M-CK

Background

Creatine kinase (CK) is a 40 kDa cytoplasmic enzyme that is integral to energy metabolism in striated muscle through the maintenance of ATP levels. It is a dimeric protein composed of tissue specific M (muscle) and B (brain) subunits. The homodimeric CKMM isozyme is predominantly expressed in differentiated skeletal and cardiac muscle fibers. The M subunit of human creatine kinase shares 97% aa sequence identity with the mouse and rat M subunit and 81% with the human B subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CKM