Creatine Kinase, Muscle/CKMM Antibody (1E3)

Western Blot: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - CKM monoclonal antibody (M02), clone 1E3 Analysis of CKM expression in IMR-32.

Immunocytochemistry/Immunofluorescence: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - Analysis of monoclonal antibody to CKM on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - Creatine Kinase, Muscle/CKMM Antibody (1E3) [H00001158-M02] - Detection limit for recombinant GST tagged CKM is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Creatine Kinase, Muscle/CKMM Antibody (1E3) Summary

• Immunogen: CKM (NP_001815, 282 a.a. ~ 381 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
• Specificity: CKM - creatine kinase, muscle
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CKM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Creatine Kinase, Muscle/CKMM Antibody (1E3)

• CKM
• CKMM
• CKMMcreatine kinase M-type
• Creatine Kinase Isoenzyme
• Creatine kinase M chain
• Creatine Kinase MM
• creatine kinase, muscle
• creatine kinase-M
• EC 2.7.3
• EC 2.7.3.2
• M-CK

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CKM
• Entrez:
  • 1158(Human)
• OMIM:
  • 123310(Human)
• Uniprot:
  • NP_001815()