![Western Blot: CPR8 Antibody [NBP3-32116] - Western blot analysis of CPR8 on different lysates with Rabbit anti-CPR8 antibody (NBP3-32116) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: THP-1 cell lysate Lane 5: SW480 cell lysate Lane 6: HEK-293 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32116) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32116_rabbit-cpr8-pab-278202415295542.jpg "Western Blot: CPR8 Antibody [NBP3-32116] - Western blot analysis of CPR8 on different lysates with Rabbit anti-CPR8 antibody (NBP3-32116) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: THP-1 cell lysate Lane 5: SW480 cell lysate Lane 6: HEK-293 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32116) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit CPR8 Antibody (NBP3-32116) is a polyclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein within human CPR8 aa 241-390 / 757. (Uniprot: Q9ULG6) |
| Localization | Cytoplasmic granule membrane. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCPG1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 87 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CPR8 Antibody (NBP3-32116)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CCPG1 |
![Flow Cytometry: CPR8 Antibody [NBP3-32116] - Flow cytometric analysis of SW480 cells labeling CPR8. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32116, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32116_rabbit-cpr8-pab-29202416453250.jpg "Flow Cytometry: CPR8 Antibody [NBP3-32116] - Flow cytometric analysis of SW480 cells labeling CPR8. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32116, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")