COX-2 Antibody [Alexa Fluor® 532]

• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

COX-2 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human COX-2
  Ala18-Leu604
  Accession # P35354
• Specificity: Detects human and mouse COX-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human COX-1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H₂. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Mature human COX-2 shows 87% aa identity to mouse COX-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.