Coronin-1a Antibody (OTI1A5) - Azide and BSA Free

Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.

Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.

Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.

Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Coronin-1a antibody, and then analyzed by flow cytometry.

CyTOF-ready: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Coronin-1a Antibody (OTI1A5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human CORO1A(NP_009005) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CORO1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 50.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Coronin-1a Antibody (OTI1A5) - Azide and BSA Free

• CLIPINA
• clipin-A
• CORO1
• coronin, actin binding protein, 1A
• coronin, actin-binding protein, 1A
• coronin-1
• coronin-1A
• Coronin-like protein A
• Coronin-like protein p57
• FLJ41407
• HCORO1
• MGC117380
• p57
• TACOCLABP
• Tryptophan aspartate-containing coat protein

Background

Coronin was originally discovered in Dictyostelium, where it was found to be involved in the chemotactic response of these ameboid cells. The name derives from the fact that the protein is localized at the leading edge or crown of these highly motile cells. The name derives from Corona, which is latin for crown. Coronin homologues have been found in yeast, C. elegans, Drosophila and many other species, and a family them are known in mammals. All coronins belong to the WD40 or WD family of proteins, the prototype or which is the beta subunit of trimeric G-proteins. Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c. The mammalian coronin family members are abundant components of eukaryotic cells, and each type has a restricted cell type specific expression pattern. Coronin 1A is the mammalian coronin most similar in protein sequence to the Dictyostelium protein and is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. NB 110-58867 is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CORO1A