Connexin 26/GJB2 Antibody

Images

 
Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). ...read more
Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa ...read more
Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Human Colon (A), Heart (B), Prostate (C), Skin (D) and negative control Adipose (E) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Connexin 26/GJB2 Antibody Summary

Immunogen
Peptide with sequence YLLIRYCSGKSKKP corresponding to C-Terminus according to NP_003995.2.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GJB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 1 ug/mL
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-57840.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Connexin 26/GJB2 Antibody

  • beta 2, 26kD (connexin 26)
  • Connexin 26
  • CX26
  • CX26DFNA3
  • DFNA3
  • DFNA3A
  • DFNB1
  • gap junction protein, beta 2, 26kDa (connexin 26)
  • gap junction protein, beta 2, 26kDa
  • GJB2
  • HID
  • KID
  • NSRD1
  • PPK

Background

Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (MIM 121014) is designated alpha-1 gap junction protein, whereas CX32 (GJB1; MIM 304040) and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Connexin 26/GJB2 Antibody (NB100-57840)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GJB2
Entrez
Uniprot