![Western Blot: Cofilin Antibody (S06-2C0) [NBP3-15015] - Western blot detection of Cofilin in A549, MCF-7, 293T cell lysates using Cofilin Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Western-Blot-NBP3-15015-img0001.jpg "Western Blot: Cofilin Antibody (S06-2C0) [NBP3-15015] - Western blot detection of Cofilin in A549, MCF-7, 293T cell lysates using Cofilin Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S06-2C0 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human Cofilin (Uniprot#: P23528) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CFL1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cofilin Antibody (NBP3-15015)Find related products by research area.
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ACTB - an abundant cytoskeletal component with applications for gene expression analysis Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi... Read full blog post. |
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| Gene Symbol | CFL1 |
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunocytochemistry of Cofilin (green) in hela using Cofilin Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Immunocytochemistry-Immunofluorescence-NBP3-15015-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunocytochemistry of Cofilin (green) in hela using Cofilin Rabbit mAb at dilution 1/50, and DAPI (blue)")
![Immunohistochemistry: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunohistochemical of Cofilin in Human lung cancer tissue using Cofilin antibody at dilution 1/2](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Immunohistochemistry-NBP3-15015-img0003.jpg "Immunohistochemistry: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunohistochemical of Cofilin in Human lung cancer tissue using Cofilin antibody at dilution 1/2")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")