Cofilin Antibody (1093310) [Unconjugated]

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CFL1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

CFL1 was detected in immersion fixed paraffin-embedded sections of liver using Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CFL1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (PC‑3) (NBP2-49856) and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for CFL1 at approximately 24 kDa (as indicated) using 10-100 µg/mL of Mouse Anti-Human CFL1 Monoclonal Antibody (Catalog # MAB11669). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clone: 1093310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cofilin Antibody (1093310) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # P23528
• Specificity: Detects a synthetic peptide specific for human CFL1 around aa 30 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 10-100 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cofilin Antibody (1093310) [Unconjugated]

• 18 kDa phosphoprotein
• CFL
• CFL1
• cofilin 1 (non-muscle)
• cofilin
• Cofilin, non-muscle isoform
• cofilin-1
• HEL-S-15
• p18

Background

Cofilin-1 (CFL1) is an 18.5 kDa protein that is a member of the actin deploy family (ADF). It is involved in the biological activities of various malignancies, including infiltration, metastasis, and chemotherapy resistance. Overexpression of CFL is associated with aggressive features and poor prognosis in malignancies. CFL1 also plays a crucial role in B cell, T cell infiltration, cytokine and NF-kappa B signaling pathways and can serve as a useful biomarker for diagnosis of inflammatory diseases.  

1. Yin X, Li X, Jiang H, Lin X, Ma Z, Chen X, Teng Q, Zhang J, Jin J. CFL1 is Implicated in Chronic Myeloid Leukemia Response during Imatinib Therapy. J Cancer. 2024 Mar 4;15(8):2424-2430. doi: 10.7150/jca.92202. PMID: 38495482; PMCID: PMC10937266. 
2. Xing J, Wang Y, Peng A, Li J, Niu X, Zhang K. The role of actin cytoskeleton CFL1 and ADF/cofilin superfamily in inflammatory response. Front Mol Biosci. 2024 Jul 24;11:1408287. doi: 10.3389/fmolb.2024.1408287. PMID: 39114368; PMCID: PMC11303188.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P23528(Human)