Monkey Coagulation Factor XI ELISA Kit (Colorimetric)

ELISA: Monkey Coagulation Factor XI ELISA Kit (Colorimetric) [NBP2-82395] - Samples were spiked with high concentrations of Monkey Coagulation Factor XI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Monkey Coagulation Factor XI ELISA Kit (Colorimetric) [NBP2-82395] - Standard Curve Reference

• Reactivity: Pm
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Monkey Coagulation Factor XI ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Monkey Coagulation Factor XI in samples. No Significant cross-reactivity or interference between Monkey Coagulation Factor XI and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.5
• Intra-Assay: CV% < 5.88
• Spike Recovery: 87-102%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F11

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey Coagulation Factor XI ELISA Kit (Colorimetric)

• Coagulation Factor XI
• EC 3.4.21
• EC 3.4.21.27
• FXIPlasma thromboplastin antecedent
• MGC141891
• PTA

Background

Factor XI encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Coagulation Factor XI ELISA Kit (NBP2-82395). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F11