Home » CLIC3 » CLIC3 Lysates » CLIC3 Overexpression Lysate (Denatured)

CLIC3 Overexpression Lysate (Denatured)

 Submit a Review

Images

 
There are currently no images for CLIC3 Lysate (H00009022-T02).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

100% Guarantee on every product

CLIC3 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-CLIC3 full-length
Gene
CLIC3

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for CLIC3

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-CLIC3 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CLIC3 Overexpression Lysate (Denatured)

  • chloride intracellular channel 3
  • chloride intracellular channel protein 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

CLIC1 Antibody
(2D4)
H00001192-M01
Western Blot: CLIC1 Antibody (2D4) [H00001192-M01] - CLIC1 monoclonal antibody (M01), clone 2D4 Analysis of CLIC1 expression in HL-60.
Immunocytochemistry/Immunofluorescence: CLIC1 Antibody (2D4) [H00001192-M01] - Analysis of monoclonal antibody to CLIC1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CLIC1 Antibody (2D4) [H00001192-M01] - Analysis of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.
(2 Publications)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
CLIC5 Antibody
(1E6)
H00053405-M03
Immunocytochemistry/Immunofluorescence: CLIC5 Antibody (1E6) [H00053405-M03] - Analysis of monoclonal antibody to CLIC5 on HeLa cell. Antibody concentration 20 ug/ml
Immunohistochemistry-Paraffin: CLIC5 Antibody (1E6) [H00053405-M03] - Analysis of monoclonal antibody to CLIC5 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
(1 Publication)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
CLIC4 Antibody
NBP1-85574
Western Blot: CLIC4 Antibody [NBP1-85574] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining in human kidney and skeletal muscle tissues using anti-CLIC4 antibody. Corresponding CLIC4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CLIC4 Antibody [NBP1-85574] - Staining of human kidney shows high expression.
(3 Publications)
Species: Hu
Applications: WB, IHC, IHC-P

Transthyretin/Prealbum ...
NBP2-52575
Western Blot: Transthyretin/Prealbumin Antibody (2E10C5) [NBP2-52575] - Analysis using TTR mAb against human TTR (AA: 1-147) recombinant protein. (Expected MW is 45.8 kDa)
Immunohistochemistry-Paraffin: Transthyretin/Prealbumin Antibody (2E10C5) [NBP2-52575] - Analysis of paraffin-embedded liver cancer tissues using TTR mouse mAb with DAB staining.
Flow Cytometry: Transthyretin/Prealbumin Antibody (2E10C5) [NBP2-52575] - Analysis of A549 cells using TTR mouse mAb (green) and negative control (red).
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
AP3B1 Antibody
NBP1-89228
Immunocytochemistry/Immunofluorescence: AP3B1 Antibody [NBP1-89228] - Staining of human cell line HeLa shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NEU3 Antibody
21630002
ELISA: NEU3 Antibody [21630002]
(2 Publications)
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
EGFR Antibody
[Unconjugated]
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
(7 Publications)
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
Rab25 Antibody
(3F12)
NBP1-51599
Western Blot: Rab25 Antibody (3F12) [NBP1-51599] - Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunocytochemistry/Immunofluorescence: Rab25 Antibody (3F12) [NBP1-51599] - Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) [NBP1-51599] - Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Prolyl
Oligopeptidase/PREP A
NBP2-33950
Western Blot: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody stainin is shown in green.
Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Staining in human colon and pancreas tissues using anti-PREP antibody. Corresponding PREP RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
gp96/HSP90B1/GRP94
Antibody (9G10)
NB300-619
Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.
Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 using Glucose-Regulated Protein 94 Monoclonal Antibody (9G10) shows staining in NIH-3T3 Cells. Glucose-Regulated Protein 94 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucose-Regulated Protein 94 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
Species: Hu, Mu, Rt, Bv, Ch, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP
CLIC2 Antibody
NBP2-32516
Western Blot: CLIC2 Antibody [NBP2-32516] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: CLIC2 Antibody [NBP2-32516] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Species: Hu
Applications: WB, IHC, IHC-P
Tyrosine
Hydroxylase Antibody
NB300-109
Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. Image from confirmed customer review.
Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Analysis of Tyrosine Hydroxylase in rat mesenteric artery-whole. Image from verified customer review.
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Immunohistochemical staining of retina tissue using TH antibody, NB300-109.
(6 Reviews)
(77 Publications)
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl
P2X2/P2RX2 Antibody
NBP2-19655
Western Blot: P2X2 Antibody [NBP2-19655] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: P2X2 Antibody [NBP2-19655] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: P2X2/P2RX2 Antibody [NBP2-19655] - Paraffin-embedded mouse fore brain. P2X2 antibody [C3], C-term dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
DDO Antibody
NBP2-32684
Immunocytochemistry/Immunofluorescence: DDO Antibody [NBP2-32684] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684] - Staining in human kidney and stomach tissues using anti-DDO antibody. Corresponding DDO RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: DDO Antibody [NBP2-32684] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PYK2/FAK2 Antibody
(SC06-15)
NBP2-67627
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse brain tissue using anti-PYK2 antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
RAB33A Antibody
NBP2-31793
Immunocytochemistry/Immunofluorescence: RAB33A Antibody [NBP2-31793] - Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.
Immunohistochemistry-Paraffin: RAB33A Antibody [NBP2-31793] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SOX5 Antibody
AF5286
Western blot shows lysates of human lung tissue and exponentially growing LNCaP human prostate cancer cell line whole cell extract. PVDF membrane was probed with 1 µg/mL of Human SOX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5286) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
GAPDH Antibody
(1D4)
NB300-221
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH, NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green) and Neurofilament Heavy antibody NB300-217 (red), counterstained with a fluorescent DNA probe (blue).
(16 Reviews)
(243 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
Bcl G Antibody
NBP1-91697
Western Blot: Bcl G Antibody [NBP1-91697] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: Bcl G Antibody [NBP1-91697] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: Bcl G Antibody [NBP1-91697] - Staining in human rectum and pancreas tissues using anti-BCL2L14 antibody. Corresponding BCL2L14 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
CLIC3
Overexpression Lysate
H00009022-T02
CLIC3 Overexpression Lysate (Denatured)
Species: Hu
Applications: WB

Publications for CLIC3 Lysate (H00009022-T02) (0)

There are no publications for CLIC3 Lysate (H00009022-T02).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CLIC3 Lysate (H00009022-T02) (0)

There are no reviews for CLIC3 Lysate (H00009022-T02). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CLIC3 Lysate (H00009022-T02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CLIC3 Products

Bioinformatics Tool for CLIC3 Lysate (H00009022-T02)

Discover related pathways, diseases and genes to CLIC3 Lysate (H00009022-T02). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CLIC3 Lysate (H00009022-T02)

Discover more about diseases related to CLIC3 Lysate (H00009022-T02).
 

Pathways for CLIC3 Lysate (H00009022-T02)

View related products by pathway.

PTMs for CLIC3 Lysate (H00009022-T02)

Learn more about PTMs related to CLIC3 Lysate (H00009022-T02).

Blogs on CLIC3

There are no specific blogs for CLIC3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

12.5% SDS-PAGE Stained with Coomassie Blue.
Recombinant Human CLIC3 Protein
H00009022-Q01-10ug
Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.
GAPDH Antibody (1D4)
NB300-221
Western Blot: CLIC3 Antibody [NBP1-89465] - Analysis in human cell lines MCF-7 and A-549 using anti-CLIC3 antibody. Corresponding CLIC3 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
CLIC3 Antibody
NBP1-89465
Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. Image from confirmed customer review.
Tyrosine Hydroxylase Antibody
NB300-109

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CLIC3 Overexpression Lysate (Denatured) and receive a gift card or discount.


Bioinformatics

Gene Symbol CLIC3