CLEC9a Antibody (7H11) [Phycoerythrin]

CD4-CD8+mouse splenocytes were stained with Hamster Anti-Mouse CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB69501A) and either (A) Rat Anti-Mouse CLEC9a PE-conjugated Monoclonal Antibody (Catalog # FAB67761P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

CLEC9a Antibody (7H11) [Phycoerythrin] Summary

• Immunogen: RBL‑2H3 rat basophilic leukemia cell line expressing mouse CLEC9a fused to an HA epitope
  Accession # Q8BRU4
• Specificity: Detects mouse CLEC9a in flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CLEC9A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLEC9a Antibody (7H11) [Phycoerythrin]

• CD370
• CLEC9a
• C-type lectin domain family 9 member A
• C-type lectin domain family 9, member A
• DNGR1
• HEEE9341
• UNQ9341

Background

CLEC9a (C-type Lectin domain family 9 member A), also known as DNGR-1, is a 44-48 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as either a disulfide-linked homodimer or monomer. Human CLEC9a expression is restricted to a subpopulation of BDCA-3⁺ conventional dendritic cells (cDC) and CD16- monocytes. In mouse, the human BDCA-3⁺ cDC are analogous to mouse CD8⁺ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In addition, mouse CLEC9a is also expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I molecules and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk, indicating that this is an activating receptor. Alternative splicing of mouse CLEC9a generates isoforms with deletions in the transmembrane segment, stalk region, or CTLD. Within aa 57-264 of the ECD, mouse CLEC9a shares 57% and 80% aa sequence identity with human and rat CLEC9a, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC9A
• Uniprot:
  • Q8BRU4(Mouse)