CLEC10A/CD301 Antibody (CL15523) - Azide and BSA Free

Analysis in human lymph node and placenta tissues using NBP3-44334 antibody. Corresponding CLEC10A/CD301 RNA-seq data are presented for the same tissues.

Staining of human liver shows moderate cytoplasmic and membranous positivity in hepatocytes.

Staining of human colon shows strong membranous positivity in lymphoid cells.

Staining of human lymph node shows strong membranous positivity in non-germinal center cells.

Staining of human placenta shows no positivity in trophoblastic cells as expected.

Analysis in control (vector only transfected HEK293T lysate) and CLEC10A/CD301 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) .

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL15523
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CLEC10A/CD301 Antibody (CL15523) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q8IUN9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC10A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for CLEC10A/CD301 Antibody (CL15523) - Azide and BSA Free

• CD301 antigen
• CD301
• CD301C-type lectin domain family 10 member A
• CLEC10A
• CLECSF13
• CLECSF14
• CLECSF14macrophage C-type lectin
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived)
• C-type lectin domain family 10, member A
• C-type lectin superfamily member 14
• HML2
• HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived)
• macrophage lectin 2 (calcium dependent)
• Macrophage lectin 2
• MGL

Background

CD301 encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC10A