CLEC10A/CD301 Antibody (CL15513) [Unconjugated] - BSA Free

Staining of human placenta shows no positivity in trophoblastic cells as expected.

Staining of human cerebral cortex shows moderate membranous-cytoplasmic positivity in endothelial cells and neuropil.

Analysis in control (vector only transfected HEK293T lysate) and CLEC10A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Staining of human rectum shows strong membranous-cytoplasmic positivity in lymphoid cells.

Analysis in human tonsil and placenta tissues using NBP3-43672 antibody. Corresponding CLEC10A RNA-seq data are presented for the same tissues.

Staining of human tonsil shows strong membranous-cytoplasmic positivity in non-germinal center cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL15513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CLEC10A/CD301 Antibody (CL15513) [Unconjugated] - BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q8IUN9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC10A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2, 40% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CLEC10A/CD301 Antibody (CL15513) [Unconjugated] - BSA Free

• CD301 antigen
• CD301
• CD301C-type lectin domain family 10 member A
• CLEC10A
• CLECSF13
• CLECSF14
• CLECSF14macrophage C-type lectin
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived)
• C-type lectin domain family 10, member A
• C-type lectin superfamily member 14
• HML2
• HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived)
• macrophage lectin 2 (calcium dependent)
• Macrophage lectin 2
• MGL

Background

CD301 encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC10A