CLCA1 Antibody

Western Blot: CLCA1 Antibody [NBP1-05038] - Analysis of CLCA1 in Human Lung lysate at 0.3ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CLCA1 Antibody Summary

• Description: Novus Biologicals Goat CLCA1 Antibody (NBP1-05038) is a polyclonal antibody validated for use in WB and ELISA. Anti-CLCA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PETPSPDETSAPC corresponding to internal region according to NP_001276.2.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLCA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in human kidney and lung lysates (calculated MW of 100 kDa band according to NP_001276.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CLCA1 Antibody

• CaCC
• CaCC-1
• Calcium-activated chloride channel family member 1
• Calcium-activated chloride channel protein 1
• chloride channel accessory 1
• chloride channel regulator 1
• chloride channel regulator
• chloride channel, calcium activated, family member 1
• CLCA1
• CLCRG1
• FLJ95147
• GOB5
• hCaCC-1
• HCLCA1

Background

This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLCA1 Antibody (NBP1-05038)(1)

Publication using NBP1-05038

• Jeulin C, Guadagnini R, Marano F. Oxidant stress stimulates Ca2+-activated chloride channels in the apical activated membrane of cultured nonciliated human nasal epithelial cells. Am J Physiol Lung Cell Mol Physiol 2005-10-01 [PMID: 16148052]

Bioinformatics

• Gene Symbol: CLCA1
• Entrez:
  • 1179(Human)
• Uniprot:
  • NP_001276.2(Human)