CLCA1 Antibody

Western Blot: CLCA1 Antibody [NB100-1791] (1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CLCA1 Antibody Summary

• Description: Novus Biologicals Goat CLCA1 Antibody (NB100-1791) is a polyclonal antibody validated for use in WB and ELISA. Anti-CLCA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TVTSKTNKDTSK corresponding to internal region according to NP_001276.1.
• Specificity: No cross-reactivity expected with CLCA2 and 3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CLCA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 80 kDa band and 30 kDa band in human duodenum lysates. These bands correspond to earlier findings in scientific literature with different antibodies (Gruber et al, Genomics. 1998 Dec 1;54(2): 200-14.). This protein has a calculated MW of 100 kDa band according to NP_001276.1.
• Control:

  Human Duodenum Whole Tissue Lysate (Adult Whole Normal)
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18314541).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CLCA1 Antibody

• CaCC
• CaCC-1
• Calcium-activated chloride channel family member 1
• Calcium-activated chloride channel protein 1
• chloride channel accessory 1
• chloride channel regulator 1
• chloride channel regulator
• chloride channel, calcium activated, family member 1
• CLCA1
• CLCRG1
• FLJ95147
• GOB5
• hCaCC-1
• HCLCA1

Background

This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLCA1 Antibody (NB100-1791)(1)

Publication using NB100-1791

• Park J, Fang S, Crews AL et al. MARCKS regulation of mucin secretion by airway epithelium in vitro: interaction with chaperones. Am J Respir Cell Mol Biol. 2008-07-01 [PMID: 18314541] (WB, Mouse)
  
  Details:
  WB (mouse NHBE cells), Fig. 2C.

Bioinformatics

• Gene Symbol: CLCA1
• Entrez:
  • 1179(Human)
• Uniprot:
  • NP_001276.1(Human)