![Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-23220_rat-clasp2-mab-kt68-18920231735526.jpg "Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | KT68 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage). |
| Immunogen | Clone KT68 was generated from a GST fusion protein containing the N-terminus of mouse CLASP2. This sequence is highly conserved in human and rat CLASP2, and has low homology to CLASP1. [UniProt# Q8BRT1] |
| Specificity | The antibody detects a 160 kDa doublet corresponding to the molecular mass of CLASP2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP2 by immunocytochemistry in HeLa and A431 cells. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | CLASP2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 160 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | CLASP2 |
![Immunocytochemistry/Immunofluorescence: CLASP2 Antibody (KT68) [NBP3-23220] - Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-CLASP2 (NBP3-23220) (middle). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23220_rat-clasp2-mab-kt68-199202312523627.jpg "Immunocytochemistry/Immunofluorescence: CLASP2 Antibody (KT68) [NBP3-23220] - Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-CLASP2 (NBP3-23220) (middle). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.")
