CL-P1/COLEC12 Antibody (388305) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clone: 388305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700

CL-P1/COLEC12 Antibody (388305) [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CL‑P1/COLEC12 isoform 1
  Leu57-Leu742
  Accession # Q5KU26
• Specificity: Detects human CL‑P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CL-P1 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CL-P1/COLEC12 Antibody (388305) [Alexa Fluor® 700]

• CL-P1
• CLP1hCL-P1
• COLEC12
• collectin placenta 1
• Collectin placenta protein 1
• collectin sub-family member 12
• collectin-12
• NSR2
• Nurse cell scavenger receptor 2
• SCARA4
• SCARA4NSR2
• Scavenger receptor class A member 4
• scavenger receptor class A, member 4
• SRCL Type I
• SRCLScavenger receptor with C-type lectin

Background

Collectins are a family of Ca⁺⁺-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewis^x epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 shares 93% aa sequence identity with mouse CL-P1 over the entire extracellular region, and 87% aa identity within each species CRD (5, 9). Human CL-P1 is expressed in vascular endothelial cells (5). CL-P1 may play a role in bacterial recognition or as a scavenger receptor for desialylated glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.