Recombinant Human CKMT2 His Protein

SDS-Page: Recombinant Human CKMT2 Protein [NBP1-98930] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human CKMT2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 40-419 of Human CKMT2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WPDARGIWHN YDKTFLIWIN EEDHTRVISM EKGGNMKRVF ERFCRGLKEV ERLIQERGWE FMWNERLGYI LTCPSNLGTG LRAGVHVRIP KLSKDPRFSK ILENLRLQKR GTGGVDTAAV ADVYDISNID RIGRSEVELV QIVIDGVNYL VDCEKKLERG QDIKVPPPLP QFGKK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CKMT2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 46.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 100 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human CKMT2 His Protein

• Basic-type mitochondrial creatine kinase
• creatine kinase S-type, mitochondrial
• creatine kinase, mitochondrial 2 (sarcomeric)
• EC 2.7.3
• EC 2.7.3.2
• mib-CK
• Sarcomeric mitochondrial creatine kinase
• SMTCK
• S-MtCK

Background

CKMT2, as known as SMTCK, belongs to the ATP:guanido phosphotransferase family. SMTCK is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. This enzyme is reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Recombinant human CKMT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CKMT2