Chromogranin B Antibody (914334) [Unconjugated]

Chromogranin B was detected in immersion fixed paraffin-embedded sections of human pituitary gland using Mouse Anti-Human Chromogranin B Monoclonal Antibody (Catalog # MAB8868) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 914334
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Chromogranin B Antibody (914334) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Chromogranin B
  Met1-Gly677
  Accession # P05060
• Specificity: Detects human Chromogranin B in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHGB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chromogranin B Antibody (914334) [Unconjugated]

• CgB
• CHGB
• chromogranin B (secretogranin 1)
• Chromogranin B
• Chromogranin-B
• SCG1
• SCG1sgI
• secretogranin B
• Secretogranin I
• secretogranin-1
• SgI

Background

Chromogranin B (CHGB), also known as Secretogranin-1, is a 673 amino acid neuroendocrine secretory granule protein which is cleaved into three fragments: PE-11 (aa 575-585), GAWK peptide (aa 440-513) and CCB peptide (617-673). Human CHGB was originally isolated from human pheochromocytoma tissue, and is expressed in normal and malignant neuroendocrine tissues. CHGB peptides play a role in hormonal regulation in pancreatic islets, hypothalamus and pituitary gland. CHGB peptides, (aa 314-332 and 350-365) have been identified in several endocrine tumors including, gastrinoma, thyroid carcinoma, insulinoma, pancreatic endocrine tumor and pituitary adenomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chromogranin B Antibody (MAB8868)(1)

Publication using MAB8868

• Yong Wang, Danlei Chen, Yingxian Pang, Xiaowen Xu, Xiao Guan, Longfei Liu Value of Immunohistochemical Expression of Apelin, Succinate Dehydrogenase B, Chromogranin B, Human Epidermal Growth Factor Receptor-2, Contactin 4, and Succinyl-CoA Synthetase Subunit Beta in Differentiating Metastatic From Non-Metastatic Pheochromocytoma and Paraganglioma Front Endocrinol (Lausanne) 2022-04-28 [PMID: 35574028]

Bioinformatics

• Gene Symbol: CHGB
• Uniprot:
  • P05060()