Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGb/7512R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human placenta stained with Chorionic Gonadotropin beta Chain (hCG beta) antibody (HCGb/7512R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using Chorionic Gonadotropin beta Chain (hCG beta) antibody (HCGb/7512R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: HCGb/7512R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGb/7512R) - Azide and BSA Free Summary

• Description: Positive Controls: Human placenta.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human Chorionic Gonadotropin beta Chain (hCG beta) protein
• Localization: Cytoplasm. Secreted.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CGB3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Chorionic Gonadotropin beta Chain (hCG beta) Antibody (HCGb/7512R) - Azide and BSA Free

• CGB
• CGB3
• CGB3choriogonadotropin subunit beta
• CGB5
• CGB7
• CGB8
• CG-Beta
• Choriogonadotropin Subunit Beta
• Chorionic gonadotrophin chain beta
• chorionic gonadotropin beta 3 subunit
• Chorionic Gonadotropin beta Chain
• chorionic gonadotropin beta subunit
• chorionic gonadotropin, beta polypeptide
• hCGB

Background

Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CGB3