CHMP2B Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

CHMP2B Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human CHMP2B
  Ala2-Asp213
  Accession # Q9UQN3
• Specificity: Detects human, mouse, and rat CHMP2B in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHMP2B Antibody [Alexa Fluor® 750]

• charged multivesicular body protein 2b
• CHMP2.5
• CHMP2.5VPS2BVPS2-2
• CHMP2B
• chromatin modifying protein 2B
• Chromatin-modifying protein 2b
• DKFZP564O123
• DMT1
• hVps2-2
• Vacuolar protein sorting-associated protein 2-2
• vacuolar protein-sorting-associated protein 2-2
• VPS2 homolog B
• Vps2-2
• VPS2B

Background

CHMP2B (CHarged Multivescular body Protein 2B; also Chromatin-Modifying Protein 2B and Vps2-2) is a 35 kDa member of the SNF family of proteins. It is a cytosolic molecule that interacts with VPS4 and undergoes polymerization to form tubules that project plasma membrane outward, generating an environment conducive to membrane fission and budding. It also appears to participate in the formation of intraluminal vesicles associated with the lysosomal system. CHMP2B is found in striated muscle (heart and skeletal), and particularly in neurons of the CNS. Human CHMP2B is 213 amino acids (aa) in length. It contains one coiled-coil region (aa 25-55), an MIT (microtubule-interacting and trafficking) domain (aa 201-211) and a utilized phosphorylation site at Ser199. There is one alternative splice form that shows a deletion of aa 2-42. Full-length human CHMP2B shares 99% aa identity with mouse CHMP2B, but only 33% aa identity with human CHMP2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.