CEP63 Overexpression Lysate

Images

 
Western Blot: CEP63 Overexpression Lysate (Adult Normal) [NBL1-09102] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CEP63.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CEP63 Overexpression Lysate Summary

Description
Transient overexpression lysate of centrosomal protein 63kDa (CEP63), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC209633 and based on accession number NM_001042400. The protein contains a C-MYC/DDK Tag.
Gene
CEP63

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
62.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CEP63 Overexpression Lysate

  • centrosomal protein 63kDa
  • centrosomal protein of 63 kDa
  • centrosome protein CEP63
  • Cep63
  • FLJ13386
  • MGC78416

Background

This gene encodes a protein with six coiled-coil domains. The protein is localized to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. Several alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform b), compared to isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-09102

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Bioinformatics

Gene Symbol CEP63