CENPN Antibody - Azide and BSA Free

Western Blot: CENPN Antibody [NBP2-92377] - Analysis of extracts of various cell lines, using CENPN at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Product Discontinued

CENPN Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human CENPN (NP_001094094). TIASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CENPN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for CENPN Antibody - Azide and BSA Free

• BM039
• C16orf60
• CENP-N
• centromere protein N
• chromosome 16 open reading frame 60
• FLJ13607
• FLJ22660
• ICEN32
• Interphase centromere complex protein 32

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on whichthe transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by thepresence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPN is anadditional factor required for centromere assembly (Foltz et al., 2006 (PubMed 16622419)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: CENPN