Recombinant Human CENPM His Protein

SDS-Page: CENPM Protein [NBP2-22766]

Product Discontinued

Recombinant Human CENPM His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-180 of Human CENPM
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVLRPL DKLPGLNTAT ILLVGTEDAL LQQLADSMLK EDCASELKVH LAKSLPLPSS VNRPRIDLIV FVVNLHSKYS LQNTEESLRH VDASFFLGKV CFLATGAGRE SHCSIHRHTV VKLAHTYQSP LLYCDLEVEG FRATMAQRLV RVLQICAGHV PGVSALNLLS LLRSSEGPSL EDL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CENPM
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human CENPM His Protein

• bK250D10.2
• C22orf18PANE1MGC861
• CENP-Mchromosome 22 open reading frame 18
• centromere protein M
• ICEN39
• Interphase centromere complex protein 39
• Pane1
• proliferation associated nuclear element 1
• Proliferation-associated nuclear element protein 1

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly. Recombinant human CENPM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CENPM