CENPM Overexpression Lysate (Native)

Images

 
Western Blot: CENPM Overexpression Lysate (Adult Normal) [NBL1-09088] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CENPM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CENPM Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201047 and based on accession number NM_024053. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens centromere protein M (CENPM), transcript variant 1, mRNA.
Gene
CENPM

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CENPM

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CENPM Overexpression Lysate (Native)

  • bK250D10.2
  • C22orf18PANE1MGC861
  • CENP-Mchromosome 22 open reading frame 18
  • centromere protein M
  • ICEN39
  • Interphase centromere complex protein 39
  • Pane1
  • proliferation associated nuclear element 1
  • Proliferation-associated nuclear element protein 1

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CENPM Lysate (NBL1-09088) (0)

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Bioinformatics

Gene Symbol CENPM