CENPM Antibody

Immunocytochemistry/Immunofluorescence: CENPM Antibody [NBP2-34079] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CENPM Antibody [NBP2-34079] - Staining in human lymph node and skeletal muscle tissues using anti-CENPM antibody. Corresponding CENPM RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: CENPM Antibody [NBP2-34079] - Staining of human lymph node shows high expression.

Immunohistochemistry-Paraffin: CENPM Antibody [NBP2-34079] - Staining of human skeletal muscle shows low expression as expected.

Product Discontinued

CENPM Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CENPM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CENPM Antibody

• bK250D10.2
• C22orf18PANE1MGC861
• CENP-Mchromosome 22 open reading frame 18
• centromere protein M
• ICEN39
• Interphase centromere complex protein 39
• Pane1
• proliferation associated nuclear element 1
• Proliferation-associated nuclear element protein 1

Background

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CENPM
• Uniprot:
  • Q9NSP4(Human)