CEBP gamma Overexpression Lysate (Native)

Images

 
Western Blot: CEBP gamma Overexpression Lysate (Adult Normal) [NBL1-09076] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CEBP gamma.

Product Details

Summary
Applications WB

Order Details

CEBP gamma Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201567 and based on accession number NM_001806. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CCAAT/enhancer binding protein (C/EBP), gamma (CEBPG), mRNA.
Gene
CEBPG

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for CEBP gamma

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CEBP gamma Overexpression Lysate (Native)

  • c/EBP gamma
  • CCAAT/enhancer binding protein (C/EBP), gamma
  • CCAAT/enhancer-binding protein gamma
  • GPE1BP
  • IG/EBP-1

Background

The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CEBP gamma Lysate (NBL1-09076) (0)

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Video Protocols

WB Video Protocol

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Blogs on CEBP gamma.

Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CEBPG

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