Human CEBP gamma ELISA Kit (Colorimetric)

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ELISA: Human CEBP gamma ELISA Kit (Colorimetric) [NBP2-75219] - Samples were spiked with high concentrations of Human CEBP gamma and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human CEBP gamma ELISA Kit (Colorimetric) [NBP2-75219] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

Order Details

Human CEBP gamma ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human C/EBP(gamma) in samples. No significant cross-reactivity or interference between Human C/EBP(gamma) and analogues was observed.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.83%
Intra-Assay
CV% < 4.72%
Spike Recovery
85-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CEBPG

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CEBP gamma ELISA Kit (Colorimetric)

  • c/EBP gamma
  • CCAAT/enhancer binding protein (C/EBP), gamma
  • CCAAT/enhancer-binding protein gamma
  • GPE1BP
  • IG/EBP-1

Background

The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP episilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for CEBP gamma ELISA Kit (NBP2-75219). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Blogs on CEBP gamma.

Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Bioinformatics

Gene Symbol CEBPG