Human CEBP gamma ELISA Kit (Colorimetric)

ELISA: Human CEBP gamma ELISA Kit (Colorimetric) [NBP2-75219] - Samples were spiked with high concentrations of Human CEBP gamma and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human CEBP gamma ELISA Kit (Colorimetric) [NBP2-75219] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL

Human CEBP gamma ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human C/EBP(gamma) in samples. No significant cross-reactivity or interference between Human C/EBP(gamma) and analogues was observed.
• Standard Curve Range: 3.13 - 200 ng/mL
• Sensitivity: 1.88 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.83%
• Intra-Assay: CV% < 4.72%
• Spike Recovery: 85-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CEBPG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CEBP gamma ELISA Kit (Colorimetric)

• c/EBP gamma
• CCAAT/enhancer binding protein (C/EBP), gamma
• CCAAT/enhancer-binding protein gamma
• GPE1BP
• IG/EBP-1

Background

The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP episilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CEBP gamma ELISA Kit (NBP2-75219). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CEBPG