CDKAL1 Antibody

Product Discontinued

CDKAL1 Antibody Summary

• Immunogen: Peptide with sequence C-QVKKQRTKDLSR corresponding to internal region according to NP_060244.2.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDKAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Has been described in a scientific literature and it has been successful usage on human islets and pancreatic insulinoma INS-1 and MIN6 cells . Preliminary experiments gave an approx. 39 kDa band in human brain (cerebellum) lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1 kDa band according to NP_060244.2. The 39 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for CDKAL1 Antibody

• CDK5 regulatory subunit associated protein 1-like 1
• CDK5 regulatory subunit-associated protein 1-like 1
• FLJ20342
• FLJ46705
• MGC75469

Background

CDKAL1 is a gene that codes for a protein with three isoforms, with lengths of 579, 488, and 97 amino acids and weights of approximately 65, 55, and 11 kDa respectively. Current studies are being done on several diseases and disorders linked to this gene including polycystic ovary syndrome, spondylitis, arterial calcification, inflammatory bowel disease, gestational diabetes, ulcerative colitis, insulin resistance, Crohn's disease, psoriasis, prostate cancer, atherosclerosis, obesity, prostatitis, pancreatitis, and malaria. CDKAL1 has also been shown to have interactions with GINS4, ATG2A, UBC, and ELAVL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDKAL1 Antibody (NBP1-36943)(1)

Publication using NBP1-36943

• Zeggini E, Weedon MN, Lindgren CM et al. Replication of genome-wide association signals in UK samples reveals risk loci for type 2 diabetes. Science 2007-06-01 [PMID: 17463249]

Bioinformatics

• Gene Symbol: CDKAL1
• Entrez:
  • 54901(Human)
  • 68916(Mouse)
  • 361243(Rat)
• Uniprot:
  • NP_060244.2(Human)