Cdk6 Antibody (1092920) [Unconjugated]

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line and SUP‑T1 human T cell lymphoblastic lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cdk6 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Cdk6 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Cdk6 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Cdk6 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western shows lysates of Jurkat human acute T cell leukemia cell line and SUP‑T1 human T cell lymphoblastic lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Cdk6 at approximately 41 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Cdk6 Monoclonal Antibody (Catalog # MAB11636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clone: 1092920
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cdk6 Antibody (1092920) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CDK-6
  Met1-Ala326
  Accession # Q00534
• Specificity: Detects recombinant human CDK-6 protein in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cdk6 Antibody (1092920) [Unconjugated]

• Cdk6
• CDKN6
• Cell division protein kinase 6
• cyclin-dependent kinase 6
• EC 2.7.11
• EC 2.7.11.22
• PLSTIREMGC59692
• Serine/threonine-protein kinase PLSTIRE

Background

Cell division protein kinase 6 (CDK6) is an enzyme that is regulated by Cyclin D proteins and Cyclin-dependent kinase inhibitor proteins. CDK6 plays an important role in hematopoiesis and loss of CDK6 causes mild anemia, thymic atrophy and delayed G1 progression in lymphocytes.  Overexpression of CDK6 has been reported in T-cell lymphoblastic lymphoma and leukemia and in all B-lymphoid malignancies.

1. Meyerson M, Harlow E. Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol. 1994 Mar;14(3):2077-86. doi: 10.1128/mcb.14.3.2077-2086.1994. PMID: 8114739; PMCID: PMC358568. 
2. Nebenfuehr S, Kollmann K, Sexl V. The role of CDK6 in cancer. Int J Cancer. 2020 Dec 1;147(11):2988-2995. doi: 10.1002/ijc.33054. Epub 2020 May 30. PMID: 32406095; PMCID: PMC7586846.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q00534(Human)