Recombinant Human CDC42EP4 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, Func, MA, AP

Recombinant Human CDC42EP4 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-356 of Human CDC42EP4 full-length ORF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MPILKQLVSSSVHSKRRSRADLTAEMISAPLGDFRHTMHVGRAGDAFGDTSFLNSKAGEPDGESLDEQPSSSSSKRSLLSRKFRGSKRSQSVTRGEREQRDMLGSLRDSALFVKNAMSLPQLNEKEAAEKGTSKLPKSLSSSPVKKANDGEGGDEEAGTEEAVPRRNGAAGPHSPDPLLDEQAFGDLTDLPVVPKATYGLKHAESIMSFHIDLGPSMLGDVLSIMDKEEWDPEEGEGGYHGDEGAAGTITQAPPYAVAAPPLARQEGKAGPDLPSLPSHALEDEGWAAAAPSPGSARSMGSHTTRDSSSLSSCTSGILEERSPAFRGPDRARAAVSRQPDKEFSFMDEEEEDEIRV

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Full Length Recombinant Protein
• Gene: CDC42EP4
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • In vitro assay
  • Protein Array
  • Western Blot
• Theoretical MW: 64.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human CDC42EP4 GST (N-Term) Protein

• BORG4MGC3740
• CDC42 effector protein (Rho GTPase binding) 4
• cdc42 effector protein 4
• CEP4Binder of Rho GTPases 4
• KAIA1777
• MGC17125

Background

The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for CDC42EP4 Recombinant Protein (H00023580-P01)(3)

Publications using H00023580-P01

• Zhao X. Identification and Characterization of Protein Kinase C Substrates in Human Breast Cells. Thesis. 2014-10-01 (In vitro)
  
  Details:
  CDC42EP4 / CEP4 protein used for in vitro kinase assay
• Xue L, Wang P, Cao P et al. Identification of ERK1 Direct Substrates using Stable Isotope Labeled Kinase Assay-Linked Phosphoproteomics. Mol Cell Proteomics. 2014-05-05 [PMID: 25022875]
• Chen X, Zhao X, Abeyweera TP, Rotenberg SA. Analysis of substrates of protein kinase C isoforms in human breast cells by the traceable kinase method Biochemistry 2012-09-11 [PMID: 22897107] (WB)

Bioinformatics

• Gene Symbol: CDC42EP4