CDC37 Antibody (JJ087-06)

Western Blot: CDC37 Antibody (JJ087-06) [NBP2-67839] - Analysis of Cdc37 on different lysates using anti-Cdc37 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: A431 Lane 3: K562

Immunohistochemistry-Paraffin: CDC37 Antibody (JJ087-06) [NBP2-67839] - Analysis of paraffin-embedded mouse placenta tissue using anti-Cdc37 antibody. Counter stained with hematoxylin.

Flow Cytometry: CDC37 Antibody (JJ087-06) [NBP2-67839] - Analysis of Hela cells with Cdc37 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunohistochemistry-Paraffin: CDC37 Antibody (JJ087-06) [NBP2-67839] - Analysis of paraffin-embedded mouse liver tissue using anti-Cdc37 antibody. Counter stained with hematoxylin.

Product Discontinued

CDC37 Antibody (JJ087-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CDC37 aa 1-50 / 378. (SwissProt: Q16543 Human; SwissProt: Q61081 Mouse; SwissProt: Q63692 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC37
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC37 Antibody (JJ087-06)

• CDC37 (cell division cycle 37, S. cerevisiae, homolog)
• CDC37 cell division cycle 37 homolog (S. cerevisiae)
• CDC37 cell division cycle 37 homolog
• CDC37A
• cell division cycle 37 homolog (S. cerevisiae)
• Hsp90 chaperone protein kinase-targeting subunit
• hsp90 co-chaperone Cdc37
• P50CDC37

Background

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC37