CD97 Isoform 1 Antibody - Isoform 1

Mouse splenocytes were stained with Goat Anti-Mouse CD97 isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3734, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD97 Isoform 1 Antibody - Isoform 1 Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD97 isoform 1
  Gln24-Arg477
  Accession # Q9Z0M6
• Specificity: Detects mouse CD97 isoform 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD97 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD97
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD97 Isoform 1 Antibody - Isoform 1

• CD97
• TM7LN1

Background

CD97 is a member of the LNB-TM7 protein family, which is a subfamily of the G-protein coupled receptor 2 family (1‑3). Molecules in this family have EGF-like modules coupled to class B G protein 7-transmembrane (TM) domains by a glycosylated (mucin) stalk. Three isoforms of CD97 are produced by alternative splicing in mouse. Isoform 1, which is the longest, contains four EGF-like domains. Only the N-terminal EGF-like domain does not bind calcium. In isoform 2, which is described in this insert, does not contain this sequence that corresponds to amino acid (aa) 120‑213 in isoform 1. Compared to isoform 1, isoform 3 lacks a 45 aa sequence between EGF-like domains 2 and 3 (4, 5). Cells known to express CD97 include monocytes, macrophages, T cells, select B cells, dendritic cells and, potentially, vascular and visceral smooth muscle cells (1, 6‑7). CD97 is also differentially expressed on murine hematopoietic stem- and progenitor-cells (7). CD55 (decay accelerating factor), a GPI-linked cell surface molecule with short consensus repeats that regulates complement activation on cell surfaces, chondroiten sulfate and the integrin alpha 5 beta (also known as VLA-5) have been identified as cellular ligands for CD97 (7). The composition of the EGF domain region defines the ligand specificity of the different CD97 isoforms (7). The first and second EGF domains interact with CD55, whereas the fourth EGF domain binds chondroitin sulfate (7). The ligand affinity of the CD97 isoforms differs (7). While affinity for CD55 is significantly higher for the smaller isoforms, chondroitin sulfate interacts exclusively with the largest isoforms (7). It has been demonstrated that CD97 is required for neutrophil migration and host defense (8).

1. McKnight, A.J. and S. Gordon (1998) J Leukoc. Biol. 63:271.
2. Stacey, M. et al. (2000) Trends Biochem. Sci. 25:284.
3. Stacey, M. et al. (2003) Blood 102:2916.
4. Hamann, J. et al. (2000) Int. Immunol. 12:439.
5. Qian, Y.-M. et al. (1999) Immunology 98:303.
6. Jaspars, L.H. et al. (2001) Tissue Antigens 57:325.
7. Van Pel, M. et al. (2008) Haematologica 93:1137.
8. Leemans, J.C. et al. (2004) J. Immunol. 172:1125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD97 Isoform 1 Antibody (AF3734)(4)

Publications using AF3734

• Hilbig D, Dietrich N, Wandel E et al. The Interaction of CD97/ADGRE5 With beta-Catenin in Adherens Junctions Is Lost During Colorectal Carcinogenesis. Front Oncol 5/25/2018 [PMID: 29888202]
• Zyryanova T, Schneider R, Adams V, Sittig D, Kerner C, Gebhardt C, Ruffert H, Glasmacher S, Hepp P, Punkt K, Neuhaus J, Hamann J, Aust G Skeletal muscle expression of the adhesion-GPCR CD97: CD97 deletion induces an abnormal structure of the sarcoplasmatic reticulum but does not impair skeletal muscle function. PLoS ONE, 2014-06-20;9(6):e100513. 6/20/2014 [PMID: 24949957]
• Karpus O, Veninga H, Hoek R, Flierman D, van Buul J, Vandenakker C, vanBavel E, Medof M, van Lier R, Reedquist K, Hamann J Shear stress-dependent downregulation of the adhesion-G protein-coupled receptor CD97 on circulating leukocytes upon contact with its ligand CD55. J Immunol, 2013-02-27;190(7):3740-8. 2013-02-27 [PMID: 23447688] (Western Blot, Mouse)
• Veninga H, Becker S, Hoek RM, Wobus M, Wandel E, van der Kaa J, van der Valk M, de Vos AF, Haase H, Owens B, van der Poll T, van Lier RA, Verbeek JS, Aust G, Hamann J Analysis of CD97 expression and manipulation: antibody treatment but not gene targeting curtails granulocyte migration. J. Immunol., 2008-11-01;181(9):6574-83. 2008-11-01 [PMID: 18941248] (IHC-Fr, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CD97
• Uniprot:
  • Q9Z0M6(Mouse)