CD97 Isoform 1 Antibody - Isoform 1

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Mouse splenocytes were stained with Goat Anti-Mouse CD97 isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3734, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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CD97 Isoform 1 Antibody - Isoform 1 Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD97 isoform 1
Gln24-Arg477
Accession # Q9Z0M6
Specificity
Detects mouse CD97 isoform 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD97 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD97
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3734 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD97 Isoform 1 Antibody - Isoform 1

  • CD97
  • TM7LN1

Background

CD97 is a member of the LNB-TM7 protein family, which is a subfamily of the G-protein coupled receptor 2 family (1‑3). Molecules in this family have EGF-like modules coupled to class B G protein 7-transmembrane (TM) domains by a glycosylated (mucin) stalk. Three isoforms of CD97 are produced by alternative splicing in mouse. Isoform 1, which is the longest, contains four EGF-like domains. Only the N-terminal EGF-like domain does not bind calcium. In isoform 2, which is described in this insert, does not contain this sequence that corresponds to amino acid (aa) 120‑213 in isoform 1. Compared to isoform 1, isoform 3 lacks a 45 aa sequence between EGF-like domains 2 and 3 (4, 5). Cells known to express CD97 include monocytes, macrophages, T cells, select B cells, dendritic cells and, potentially, vascular and visceral smooth muscle cells (1, 6‑7). CD97 is also differentially expressed on murine hematopoietic stem- and progenitor-cells (7). CD55 (decay accelerating factor), a GPI-linked cell surface molecule with short consensus repeats that regulates complement activation on cell surfaces, chondroiten sulfate and the integrin alpha 5 beta (also known as VLA-5) have been identified as cellular ligands for CD97 (7). The composition of the EGF domain region defines the ligand specificity of the different CD97 isoforms (7). The first and second EGF domains interact with CD55, whereas the fourth EGF domain binds chondroitin sulfate (7). The ligand affinity of the CD97 isoforms differs (7). While affinity for CD55 is significantly higher for the smaller isoforms, chondroitin sulfate interacts exclusively with the largest isoforms (7). It has been demonstrated that CD97 is required for neutrophil migration and host defense (8).

  1. McKnight, A.J. and S. Gordon (1998) J Leukoc. Biol. 63:271.
  2. Stacey, M. et al. (2000) Trends Biochem. Sci. 25:284.
  3. Stacey, M. et al. (2003) Blood 102:2916.
  4. Hamann, J. et al. (2000) Int. Immunol. 12:439.
  5. Qian, Y.-M. et al. (1999) Immunology 98:303.
  6. Jaspars, L.H. et al. (2001) Tissue Antigens 57:325.
  7. Van Pel, M. et al. (2008) Haematologica 93:1137.
  8. Leemans, J.C. et al. (2004) J. Immunol. 172:1125.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD97
Uniprot