CD79B Antibody (HM79-12)

Images

 
Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the PE conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.
Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - 1 ug/106 BALB/c splenocytes were incubated with either hamster IgG (as an isotype control) or purified hamster anti-mouse CD79, and then stained with mouse ...read more
Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the Biotin conjugate of NBP1-28140. Multiple staining of C57BL/6 splenocytes.
Flow Cytometry: CD79B Antibody (HM79-12) [NBP1-28140] - Analysis using the FITC conjugate of NBP1-28140. Staining of C57BL/6 splenocytes.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
Clone
HM79-12
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

CD79B Antibody (HM79-12) Summary

Immunogen
CD79alpha/CD79B heterodimers purified from mouse (BALB/c x NZB)F1 B cell lymphoma cells, WEHI231
Specificity
beta chain of the mouse B-cell receptor, Mr 23 kDa
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD79B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Publications
Read Publications using
NBP1-28140 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD79B Antibody (HM79-12)

  • B29
  • B29B-cell-specific glycoprotein B29
  • B-cell antigen receptor complex-associated protein beta chain
  • CD79B antigen (immunoglobulin-associated beta)
  • CD79b antigen
  • CD79b molecule, immunoglobulin-associated beta
  • CD79B
  • IGB
  • IGBAGM6
  • Ig-beta
  • Immunoglobulin-associated B29 protein

Background

Murine CD79 is a 23/21 kDa disulfide-linked heterodimer composed of an alpha chain (CD79alpha/mb-1) and a beta chain (CD79b/B29) that associates non-covalently with membrane immunoglobulin (Ig) to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the pro-B cell stage prior to productive Ig gene rearrangements and remaining through all stages of B-cell differentiation prior to plasma cells. It has been proposed that the CD79 complex on pro-B cell surfaces may function to induce early B-cell differentiation. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD79B Antibody (NBP1-28140)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Mouse
(2)
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Showing Publications 1 - 4 of 4.
Publications using NBP1-28140 Applications Species
Koyama M, Ishihara K, Karasuyama H et al. CD79a/CD79b heterodimers are expressed on pro-B cell surfaces without associated m heavy chain International Immunology

Details:
This publication used the FITC conjugated form of this antibody (Cat# NBP1-28141).
Chen Y, Zheng Y, You X et al. Kras Is Critical for B Cell Lymphopoiesis J Immunol (WB, Mouse)

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-28142).
WB Mouse
Chen Y, Zheng Y, You X et al. Kras Is Critical for B Cell Lymphopoiesis J Immunol 2016 Jan 15 [PMID: 26773157] (WB, Mouse)

Details:
This citation used the Biotin version of this antibody.
WB Mouse
Mariko Koyama, Katsuhiko Ishihara, Hajime Karasuyama et al. CD79a/CD79b heterodimers are expressed on pro-B cell surfaces without associated m heavy chain International Immunology 1997 Nov [PMID: 9418137]

Details:
This citation used the FITC version of this antibody.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28142
FITC Labeled NBP1-28141
PE Labeled NBP1-28143

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Blogs on CD79B.

CD79b - A Signal Transduction Component of the B-cell Receptor
The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway...  Read full blog post.

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Bioinformatics

Gene Symbol CD79B
Entrez