Home » CD79B » CD79B Antibodies » CD79B Antibody (HM79-11) [FITC]

CD79B Antibody (HM79-11) [FITC]

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Flow Cytometry: CD79B Antibody (HM79-11) [FITC] [NB100-64017] - Staining of mouse spleen cells with HAMSTER ANTI MOUSE CD79b:FITC
Flow Cytometry: CD79B Antibody (HM79-11) [FITC] [NB100-64017] - Staining of mouse spleen cells with HAMSTER ANTI MOUSE CD79b:FITC

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
HM79-11
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
FITC
Concentration
0.1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65611AF350
Alexa Fluor 405
NB100-65611AF405
Alexa Fluor 488
NB100-65611AF488
Alexa Fluor 532
NB100-65611AF532
Alexa Fluor 594
NB100-65611AF594
Alexa Fluor 647
NB100-65611AF647
Alexa Fluor 700
NB100-65611AF700
Alexa Fluor 750
NB100-65611AF750
Allophycocyanin
NB100-65611APC
Allophycocyanin/Cy7
NB100-65611APCCY7
DyLight 350
NB100-65611UV
DyLight 405
NB100-65611V
DyLight 488
NB100-65611G
DyLight 550
NB100-65611R
DyLight 594
NB100-65611DL594
DyLight 650
NB100-65611C
DyLight 680
NB100-65611FR
DyLight 755
NB100-65611IR
Janelia Fluor 549
NB100-65611JF549
Janelia Fluor 646
NB100-65611JF646
PE
NB100-65611PE
PE/Atto594
NB100-65611PEATT594
PE/Cy5.5
NB100-65611PECY55
PE/Cy7
NB100-65611PECY7
PerCP
NB100-65611PCP
Unconjugated
NB100-65611
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CD79B Antibody (HM79-11) [FITC] Summary

Immunogen
CD79 alpha/CD79 beta heterodimer purified from WEH1231 B cells.
Specificity
NB100-65611 recognizes murine CD79 beta, which is expressed by B cells as part of the B cell receptor complex (immunoglobulin and the CD79 alpha/beta heterodimer). Murine CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin, and is expressed throughout B cell differentiation. CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD79B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry Neat
Publications
Read Publication using NB100-64017.

Reactivity Notes

Reacts with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for CD79B Antibody (HM79-11) [FITC]

  • B29
  • B29B-cell-specific glycoprotein B29
  • B-cell antigen receptor complex-associated protein beta chain
  • CD79B antigen (immunoglobulin-associated beta)
  • CD79b antigen
  • CD79b molecule, immunoglobulin-associated beta
  • CD79B
  • IGB
  • IGBAGM6
  • Ig-beta
  • Immunoglobulin-associated B29 protein

Background

CD79b is the B cell antigen receptor Ig beta chain (BCR Ig beta), which associates with B cell antigen receptor Ig alpha chain to form a functional heterodimer that interacts with membrane immunoglobulin (mIg). The mIgM component binds antigen and the Ig alpha/Ig beta heterodimer tranduces signals. CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.
Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human BCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5129) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BCR at approximately 155 - 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.
(3 Reviews)
(6 Publications)
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CD38 Antibody
(1G7F4)
NBP2-25250
Simple Western: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis using Azide Free version of NBP2-25250. Lane view shows a specific band for CD38 in 0.2 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Frozen: CD38 Antibody (1G7F4) [NBP2-25250] - B cell lymphoma tumor sections (Ramos). Antibody Dilution 1:200. IHC-Fr image submitted by a verified customer review.
Western Blot: CD38 Antibody (1G7F4) [NBP2-25250] - Analysis of (A) Human Jurkat, (B) Human Ramos and (C) Mouse EL4 lysate using this antibody at 4 ug/mL.
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD79B Antibody (NB100-64017)(1)


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-65611AF350
Alexa Fluor 405 NB100-65611AF405
Alexa Fluor 488 NB100-65611AF488
Alexa Fluor 532 NB100-65611AF532
Alexa Fluor 594 NB100-65611AF594
Alexa Fluor 647 NB100-65611AF647
Alexa Fluor 700 NB100-65611AF700
Alexa Fluor 750 NB100-65611AF750
Allophycocyanin NB100-65611APC
Allophycocyanin/Cy7 NB100-65611APCCY7
DyLight 350 NB100-65611UV
DyLight 405 NB100-65611V
DyLight 488 NB100-65611G
DyLight 550 NB100-65611R
DyLight 594 NB100-65611DL594
DyLight 650 NB100-65611C
DyLight 680 NB100-65611FR
DyLight 755 NB100-65611IR
FITC NB100-64017
Janelia Fluor 549 NB100-65611JF549
Janelia Fluor 646 NB100-65611JF646
PE NB100-65611PE
PE/Atto594 NB100-65611PEATT594
PE/Cy5.5 NB100-65611PECY55
PE/Cy7 NB100-65611PECY7
PerCP NB100-65611PCP
Learn about Custom Antibody Labeling

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Bioinformatics Tool for CD79B Antibody (NB100-64017)

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Blogs on CD79B.

Check out the latest blog posts on CD79B.
CD79b - A Signal Transduction Component of the B-cell Receptor
The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway...  Read full blog post.
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
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Bioinformatics

Gene Symbol CD79B