CD39/ENTPD1 Antibody (A1)

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with CD19 Monoclonal Antibody and either (top) Mouse Anti-Human CD39 (catalog #NBP3-48409) or (bottom) matched isotype control antibody.

CD39 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD39 Monoclonal Antibody (Catalog #NBP3-48409) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD39/ENTPD1 Antibody (A1) Summary

• Immunogen: PHA activated human lymphocytes
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENTPD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD39/ENTPD1 Antibody (A1)

• ADPase
• ATPDase
• ATP-diphosphatase
• CD39 antigen
• CD39
• CD39EC 3.6.1.5
• DKFZp686D194
• DKFZp686I093
• EC 3.6.1
• ecto-apyrase
• Ecto-ATP diphosphohydrolase 1
• Ecto-ATPase 1
• Ecto-ATPDase 1
• ectonucleoside triphosphate diphosphohydrolase 1
• ENTPD1
• FLJ40921
• FLJ40959
• Lymphoid cell activation antigen
• NTPD1
• NTPDase 1
• NTPDase-1

Background

CD39, also known as ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1), is the prototypical member of the NTPDase family. CD39 is a metal-dependent, homodimeric enzyme bound to the cell membrane through two transmembrane domains present at each termini (1-3). The extracellular loop contains two major domains with an active site and apyrase conserved regions (ACRs) and changes conformation dynamically during catalysis (1-4). Although first described as a B lymphocyte cell surface marker (5), CD39 is also present on the surface of natural killer cells, T cells, and some endothelial cells (6). CD39 hydrolyzes the beta - and gamma  phosphate residues of nucleotides to terminate purinergic signaling and is uniquely capable of processive hydrolysis of extracellular ATP to AMP without release of the ADP intermediate (3, 7, 8) as the initial rate-limiting steps in the production of extracellular adenosine (9). CD39 functional activity leads to interest in CD39 as a pharmaceutical therapeutic target for several disease models including inflammation, cancer, and immunosuppression (3,4,9,10). Recombinant cynomolgus NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble recombinant NTPDase-1 ectodomain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENTPD1