CD300a/LMIR1 Antibody (C4) - BSA Free

Western Blot: CD300a/LMIR1 Antibody (C4) [NBP3-41101] - Sample: Porcine Lymph node lysate Primary Ab: 0.4ug/ml NBP3-41101; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

• Reactivity: Mu, Po
• Applications: WB
• Clone: C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD300a/LMIR1 Antibody (C4) - BSA Free Summary

• Description: Novus Biologicals Mouse CD300a/LMIR1 Antibody (C4) - BSA Free (NBP3-41101) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Immunized with recombinant Mouse CD300a/LMIR1 (Uniprot#: Q6SJQ0; Gly39-Gln284 (Uniprot#: Q6SJQ0)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD300A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01M PBS, pH7.4, 50% glycerol
• Preservative: 0.05% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD300a/LMIR1 Antibody (C4) - BSA Free

• CD300a antigenCMRF35H9
• CD300a molecule
• CD300a
• CLM8
• CLM-8
• CMRF35H leukocyte immunoglobulin-like receptor
• CMRF-35H
• CMRF35-H
• CMRF35-H9
• CMRF-35-H9CD300 antigen-like family member A
• CMRF35HIRp60
• CMRF35-like molecule 8
• IGSF12
• IGSF12NK inhibitory receptor
• Immunoglobulin superfamily member 12
• Inhibitory receptor protein 60
• IRC1
• IRC1/IRC2
• IRC2
• IRp60
• leukocyte membrane antigen
• LMIR1
• MAIR-I
• Mcipr1
• NKRL
• Pigr4

Background

Human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), is a 60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells. Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD300A